In this study, analysis of the mitochondrial and Y-chromosomal haplogroups was used to provide information about the phylogenetic groups of Tutankhamun’s family members, and their presence among the reported contemporary Egyptian population data. The mitochondrial and Y-chromosomal DNA analysis of Tutankhamun’s family confrms our previous data of the royal family pedigree,with multiple controls authenticating all results. The proposed sibling relationship between Tutankhamun’s parents, KV55 (Akhenaten) and KV35YL, is further supported. The royal lineage is composed of the Y-chromosome haplogroup R1b and the mitochondrial haplogroup K. Population genetics point to a common origin at ca. 14 000-28 000 years before present locating to the Near East.
KV46 Thuya haplogroup K
KV35EL Tiye haplogroup K
KV35YL haplogroup K
KV55 Akhenaten haplogroup K
KV62 Tutankhamun haplogroup K
KV46 Yuya haplogroup K
KV35 Amenhotep III haplogroup H2b
KV60 Sitra-In not haplogroup K
KV60 Hatshepsut not haplogroup K
Yuya haplogroup G2a
Amenhotep III R1b
KV55 Akhenaten R1b
Tutankhamun R1b
TT320-CCG61065 L (Originally thought to be Thutmose I, father of Hatshepsut; an unidentified royal of the 18th dynasty)
KV46 Thuya haplogroup K
KV35EL Tiye haplogroup K
KV35YL haplogroup K
KV55 Akhenaten haplogroup K
KV62 Tutankhamun haplogroup K
KV46 Yuya haplogroup K
KV35 Amenhotep III haplogroup H2b
KV60 Sitra-In not haplogroup K
KV60 Hatshepsut not haplogroup K
Yuya haplogroup G2a
Amenhotep III R1b
KV55 Akhenaten R1b
Tutankhamun R1b
TT320-CCG61065 L (Originally thought to be Thutmose I, father of Hatshepsut; an unidentified royal of the 18th dynasty)